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Enterobacter cloacae

Enterobacter cloacae

Enterobacter cloacae on Endo agar with biochemical slope (see here). Glucose fermentation with gas production (fig.E), urea positive (about 70%) and H2S negative, lactose positive(fig.F). Sucrose, mannitol and cellobiose positive.

 

 

COLONY MORPHOLOGY

enterobacter cloacae enterobacter cloacae enterobacter cloacae enterobacter cloacae
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GRAM-NEGATIVE RODS
MOTILE
NONSPOREFORMING
CATALASE: POSITIVE
OXIDASE: NEGATIVE
FACULTATIVELY ANAEROBIC

BASIC TESTS
FOR IDENTIFICATION

-
MacConkey growth+
Indole production-
Methyl red-
Voges-Proskauer+
Citrate(Simmons)+
Hydrogen sulfide(TSI)
Urea hydrolysisD
Lysine decarboxylase-
Arginine dihydrolase+
Ornithine decarboxylase+
Motility (36 °C)+
D-glucose acid/gas+/+
D-mannitol fermentation+
Sucrose fermentation+
Lactose fermentation+
D-sorbitol fermentation+
Cellobiose+
Esculin hydrolisisd
Acetate utilizationD
ONPG test+
 
+ positive ( > 90% of strains are positive)
D most positive (51 - 89%)
d most negative (11 - 50%)
- negative (0 - 10%)

ANTIBIOTIC
TREATMENT

Should be always guided by
in vitro susceptibility
tests!!

Selection of appropriate antibiotics depends on diagnosis!!

IF SUSCEPTIBLE:

Imipenem + aminoglycoside(e.g., gentamicin or amikacin) Piperacillin/tazobactam + aminoglycoside Fluoroquinolones(e.g., ciprofloxacin, norfloxacin)
Trimethoprim-sulfamethoxazole (Co-trimoxazole)
Nitrofurantoin
Ceftazidime

ALTERNATIVES:

Cephalosporins III, IV
list of anntibiotics Gram-negative god
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Enterobacter cloacae

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