Klebsiella pneumoniae, positive tests

    Typical biochemical properties of Klebsiella pneumoniae are:

  • lactose fermentation (pink, mucous colonies on Endo; compare with E.coli lactose positive colonies)
  • D-mannitol fermentation (colour change around the tablet isn't obvious; compare with Shigella)
  • sucrose fermentation (colour change around the tablet isn't obvious)
  • urea hydrolysis
  • acid from glucose with gas
  • hydrogen sulfide negative

  • General description of testing on Endo agar with biochemical slope is here.

    Other (more or less) necessary tests for identification of Klebsiella pneumoniae are:
  • Indole production (negative)
  • Voges-Proskauer test (positive)
  • Simmons citrate (positive)
  • Esculin hydrolysis (positive)
  • Malonate utilization (positive)
  • Lysin decarboxylase (positive)
  • Arginine dihydrolase (negative)
  • Ornithine decarboxylase (negative)

  • Compare with Escherichia coli results.

    An example of Klebsiella pneumoniae identification with a commercial kit (ENTEROtest24).